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This paper gives an overview of industrial SMB chromatography and focusses on the strategy how to develop a purification system either in the early development phase of a product or to assess whether a batch process can be optimised and scaled into a continuous process.

Biotechnological fermentation processes are widely used in industry to produce an abundant range of organic products which often need to be purified in order to meet high-quality standards.
Typical bioprocesses comprise:

  • upstream (USP, e.g. prior to, and including fermentation)
  • downstream (DSP following the fermentation-harvesting, e.g. purification and crystallisation)

Industrial Bioprocesses can be characterised by a few common rules of thumb:

  • Typical fermentation broth contains many compounds (product, sugars, proteins and biomass residuals)
  • Downstream processing is necessary for purification and accountable for the highest process costs

Reducing downstream process steps can reduce capital investment and operational costs and reduce the overall energy consumption of an industrial process.


Conventional downstream processing involves biomass separation from the soluble fractions of the fermentation broth as a first step (e.g. filtration, centrifugation). Hereafter, other downstream process steps follow, depending on the required product purity and concentration. Decolorization is often necessary to remove the brownish colour of a fermentation broth, caused by degenerated sugar and proteins. Adsorption chromatography is widely used to bind the product of interest or the impurities to a specific resin (sorbens), packed in a column (packed bed). The bound product is then eluted and can be used in further processing like crystallisation. After elution, the resin/column is regenerated and cleaned (CIP-Cleaning In Place).

A typical process flow diagram is shown in the figure below.

Conventional bioprocesses can be summarised by the following steps:

  • Biomass removal (e.g. flocculation, filtration, centrifugation)
  • Decolorization
  • Adsorption Chromatography
  • Concentration and Crystallisation
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